Fermentation culture method of recombinant escherichia coli for producing insulin aspart fusion protein

The invention provides a culture medium suitable for fermenting recombinant escherichia coli to produce insulin aspart fusion protein. Specifically, the invention provides the culture medium containing yeast peptone, yeast extract powder, glycerol, Boc-L-lysine, a buffering agent and trace elements. The invention further provides a method for producing the insulin aspart fusion protein by fermentation of the culture medium. When the culture medium is used for fermentation, high bacterial density and high insulin aspart fusion protein yield can be obtained, the fermentation culture time is short, raw materials are easy to obtain, the virus carrying risk is avoided, the cost is low, and the culture medium is suitable for industrial production. 本发明提供了一种适于重组大肠杆菌发酵产门冬胰岛素融合蛋白的培养基。具体地，本发明提供了一种包含酵母蛋白胨、酵母浸粉、甘油、Boc-L-赖氨酸、缓冲剂和微量元素的培养基。本发明还提供了利用该培养基发酵生产门冬胰岛素融合蛋白的方法。利用本发明的培养基发酵，能获得较高的菌密度和较高的门冬胰岛素融合蛋白产量，且发酵培养时间短，原料易得，无病毒携带风险，成本较低，适用于工业生产。