Immunochromatography test paper and preparation method and application thereof

The invention discloses immunochromatography test paper and a preparation method and application thereof, and relates to the technical field of immunochromatography detection.The immunochromatography test paper is characterized in that streptavidin is used for conducting detection line membrane scratching, and biotinylated antibodies are transferred to a combination pad, so that double-antibody sandwich reaction of antigens and antibodies starts from the combination pad, the reaction time is prolonged, and the reaction efficiency is improved; the biotinylated antibody can flow on the nitrocellulose membrane, and the reaction capacity with the antigen is higher than that of the antibody fixed on the detection line, so that the production efficiency of the double-antibody sandwich compound is improved, and the sensitivity is improved. Meanwhile, a capture reaction on the nitrocellulose membrane is converted from an antigen-antibody reaction to a streptavidin-biotin reaction, the reaction efficiency is improved