A RIBOZYME COMPRISING A TARGET-BINDING DOMAIN

The present application discloses a ribozyme comprising a target-binding domain which is for binding of a target RNA molecule. The ribozyme further comprises one or more catalytic domains and one or more releasable RNA segments flanked by two ribozyme cleavage sites, wherein activation of the cataly...

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Bibliographische Detailangaben
Hauptverfasser:	LIM YU THENG MANDY, KOH CHONG HUI, AW SHERRY
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present application discloses a ribozyme comprising a target-binding domain which is for binding of a target RNA molecule. The ribozyme further comprises one or more catalytic domains and one or more releasable RNA segments flanked by two ribozyme cleavage sites, wherein activation of the catalytic domains by a target RNA molecule causes the cleavage at the ribozyme cleavage sites, causing the releasable RNA segment to be released from the ribozyme. The present application further discloses the use of such ribozymes for detecting the presence of a target RNA molecule or the amplification of a target RNA molecule. 本申请公开了一种包含靶结合结构域的核酶，所述靶结合结构域用于结合靶RNA分子。所述核酶进一步包含一个或多个催化结构域和侧翼为两个核酶切割位点的一个或多个可释放RNA区段，其中通过靶RNA分子的催化结构域的激活导致在核酶切割位点的切割，这导致可释放RNA区段从核酶中释放。本申请进一步公开了所述核酶用于检测靶RNA分子的存在或扩增靶RNA分子的用途。