System for site-specific modification of pAPN gene and application thereof

The invention relates to the technical field of biology, and particularly provides a system for site-specific modification of pAPN gene and application thereof. The invention provides a system for site-specific modification of a pAPN gene, a first vector and a second vector contained in the system can express gene editing protein and sgRNA, effective enzyme digestion is performed on two target sites of the pAPN gene, a site-specific modification fragment of donor DNA is used for replacing a to-be-site-specific modification fragment located between the two target sites, so that the precise mutation of the 738th amino acid of the pAPN gene is realized, and T738 is replaced by V738. When the 738th amino acid of the pAPN gene is accurately modified, and normal expression of other amino acids of the pAPN gene can be prevented from being damaged or changed, so that on the basis of resisting TGEV infection, the physiological activity function of the pAPN protein is reserved to the maximum extent, and the method has