Duck embryo primary hepatocyte separation culture method

The invention relates to the field of cell culture, in particular to a duck embryo primary hepatocyte separation culture method. The method comprises the following steps: disinfecting shells of duck embryos, and taking 13-15-day-old duck embryos under an aseptic condition; separating the liver of the duck embryo, removing gall bladder, and soaking and washing the obtained liver in a D-Hank's buffer solution; removing loose connective tissues and liver envelopes adhered to the periphery of the liver, and soaking and washing the obtained small pieces of liver tissues in D-Hank's buffer solution; performing multi-step digestion; and re-suspending the obtained cells, sieving the cells with a 70-[mu]m cell sieve, and inoculating the cells into a culture bottle for culture. According to the invention, a simple, economical and rapid duck embryo primary hepatocyte separation method is established from the selection of the day age of the duck embryo, the combination of a mechanical method and an enzyme digestion metho