Kinase enrichment detection method based on chemical proteomics

The invention provides a kinase enrichment detection method based on chemical proteomics, and belongs to the technical field of kinase detection. A plurality of small molecule inhibitors are respectively coupled on a solid carrier to carry out affinity enrichment on kinase in cells or tissues, and t...

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Bibliographische Detailangaben
Hauptverfasser: XUE BINGBING, LIU TAIYI, RUAN HONGQIANG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a kinase enrichment detection method based on chemical proteomics, and belongs to the technical field of kinase detection. A plurality of small molecule inhibitors are respectively coupled on a solid carrier to carry out affinity enrichment on kinase in cells or tissues, and the abundance and phosphorylation level of the kinase are researched in a physiological state. Kinase identification is realized through high performance liquid chromatography separation, mass spectrometry detection and database search, and large-scale unbiased analysis can be carried out on kinase. 本发明提供一种基于化学蛋白质组学的激酶富集检测方法,属于激酶检测技术领域,分别将多种小分子抑制剂偶联在固体载体上来亲和富集细胞或组织中的激酶,在生理状态下研究激酶的丰度和磷酸化水平,通过高效液相色谱分离、质谱检测和数据库搜索实现激酶的鉴定,可以对激酶进行大规模无偏差分析。