High-throughput detection method and system for antibiotic resistance gene in environmental sample

The embodiment of the invention discloses a high-throughput detection method and system for antibiotic resistance genes in an environmental sample, and the method comprises the following steps: extracting metagenome DNA of the environmental sample, sequencing and building a database to obtain metagenome data; performing quality control on the metagenome data, and then splicing to obtain a spliced sequence; performing gene prediction and protein translation on the spliced sequence to obtain a gene with a complete sequence; performing antibiotic resistance gene prediction and screening on the gene with the complete sequence to obtain a first antibiotic resistance gene, predicting the source of the first antibiotic resistance gene, extracting the sequence of a second antibiotic resistance gene of which the source is not detected, and performing extraction and source detection on the protein sequence of the second antibiotic resistance gene; and performing classification status analysis on the source information