Application of chicken CTGF gene in inhibition of chicken preadipocyte differentiation

The invention discloses application of a chicken CTGF gene in inhibition of chicken preadipocyte differentiation. The nucleotide sequence of the chicken CTGF gene is as shown in SEQ ID NO.1, and the coded amino acid sequence of the chicken CTGF gene is as shown in SEQ ID NO.2. According to the inven...

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Bibliographische Detailangaben
Hauptverfasser: LI HUI, ZHOU JIAMEI, GE YAOWEN, XIAO FAN, ZHANG HUI, SHEN LINYONG, WANG SHUPING, GAO HAIHE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses application of a chicken CTGF gene in inhibition of chicken preadipocyte differentiation. The nucleotide sequence of the chicken CTGF gene is as shown in SEQ ID NO.1, and the coded amino acid sequence of the chicken CTGF gene is as shown in SEQ ID NO.2. According to the invention, through a method for adding an exogenous CTGF recombinant protein and constructing an endogenous pCMV-CTGF overexpression vector, an oil red O technology, a Real-time PCR technology and a Western blot technology are utilized to prove that the CTGF gene can inhibit chicken preadipocytes from being differentiated into mature adipocytes from the lipid droplet deposition capability, the transcriptome level and the proteome level of the chicken adipocytes, and thus, the invention provides novel application of the CTGF gene in regulation and control of the chicken preadipocyte differentiation process, and the CTGF gene has practical value in the fields of chicken genetic breeding and animal nutrition. 本发明公开一种鸡CTGF基