RCAN1.1 promoter fragment for cell hypoxia and HIF1alpha protein activity indicator and application thereof

The invention discloses an RCAN1.1 promoter fragment for a cell hypoxia and HIF1alpha protein activity indicator and application thereof, and relates to the technical field of genetic engineering. According to the invention, dual-luciferase reporter gene detection system is adopted, an RCAN1.1-1030-...

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Bibliographische Detailangaben
Hauptverfasser:	YUN YAN, SUN XIULIAN, ZHAO JUAN, YANG XIAXIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses an RCAN1.1 promoter fragment for a cell hypoxia and HIF1alpha protein activity indicator and application thereof, and relates to the technical field of genetic engineering. According to the invention, dual-luciferase reporter gene detection system is adopted, an RCAN1.1-1030-302 promoter fragment is inserted into a blank vector pGL3-basic with a firefly luciferase reporter gene, a recombinant plasmid RCAN1.1-1030-302-LUC for expressing the firefly luciferase is constructed, the activation condition of the RCAN1.1-1030-302-LUC promoter fragment is judged by detecting the luciferase activity of the recombinant plasmid RCAN1.1-1030-302-LUC, further, the hypoxia state of the cells and the activity of the HIF1alpha protein are judged. The RCAN1.1-1030-302 promoter fragment provided by the invention can be activated by the HIF1alpha protein, has high sensitivity to the HIF1alpha protein, and can be used as a cell hypoxia and HIF1alpha protein activity indicator. 本发明公开了一种用于细胞缺氧和HIF1α蛋白活性指示剂的R