5-aminolevulinic acid producing strain and construction method thereof

The invention discloses a 5-aminolevulinic acid producing strain and a construction method thereof. The construction method comprises the following steps of (1) knocking out pxpR, PoxB, ptsG, sucC, sucD and pta genes in bacillus subtilis to obtain a bacillus subtilis engineering strain; (2) carrying out double enzyme digestion treatment on the plasmid pHT43-J by using Aat II and Sma I, integrating the hemO gene onto the plasmid pHT43-J subjected to double enzyme digestion treatment to obtain a recombinant plasmid pHT43-J-hemO, then carrying out enzyme digestion treatment on the recombinant plasmid pHT43-J-hemO by using XbaI and BamHI, and integrating the rhtA gene onto the recombinant plasmid pHT43-J-hemO subjected to enzyme digestion treatment to obtain a first recombinant expression vector; carrying out double enzyme digestion on the plasmid pHT01 by using BamHI and XbaI, and integrating the hemF gene onto the plasmid pHT01 subjected to double enzyme digestion treatment, so as to obtain a second recombinant