Passage method of streptomyces darkens strain

The invention relates to the technical field of microbial fermentation strain preparation, and discloses a passage method of a streptomyces darkness strain, which comprises streptomyces darkness spores and streptomyces darkness spores stored in a glycerol tube under the condition of -20 DEG C. Accor...

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Hauptverfasser: NIU QIULI, ZHANG GUIFANG, LI RUINA, WEI LEINA, YANG JIANYIN, WANG XINHONG, LI SHANGCHAO, LI RUIFANG, LIU YUANSHENG, ZHOU ZHIXIA, CUI GAOFEI, SONG YANJUN, SUN WEIQIANG, CHEN SHUFANG, CHONG LIHONG, SHI LIJUN, ZHENG JISHUAN, WEI JINGLI, XU XIA, PING BAOQIN, WANG YONGGUANG, LI NING, DENG HUIMIN, CHEN WENCHENG, ZHU MINGJIE, LI XUANTAO, HAN ZHENGUO, SUN CHUNLING, LI TONGTONG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the technical field of microbial fermentation strain preparation, and discloses a passage method of a streptomyces darkness strain, which comprises streptomyces darkness spores and streptomyces darkness spores stored in a glycerol tube under the condition of -20 DEG C. According to the passage method of the streptomyces darkens strain, the culture medium of the culture dish is uniform in thickness and uniform in nutritional ingredient distribution, so that single colony spores on the culture medium are under the same nutritional condition, sufficient nutritional ingredients are provided for growth of the spores, and the situation is avoided that part of the spores are insufficient in absorption of the nutritional ingredients due to inconsistent thickness on a slant culture medium, and the normal growth phenomenon of spores is influenced. By diluting and coating glycerol tube spores, single spores can separately grow into single colonies in a culture medium of a culture dish, and the f