TETRA-FUNCTIONAL CHEMICAL PROBE AND METHOD FOR IDENTIFYING TARGET MEMBRANE PROTEIN FROM LIVING CELL OR LIVING TISSUE BY USING SAID PROBE

This tetra-functional compound is formed by linking, via a spacer as needed, a ligand binding site (A) or a ligand, a reactive site (D), a potential cleavage site (E), and a biotin site (B), wherein: the ligand binding site (A) is an active functional group such as an amine reactive group or a react...

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Hauptverfasser:	HAYASHI TAKASHI, SAKAI KOJI, WADATSU TAKASHI, TANAKA MASAKI, NAGABUKURO AKIRA, NISHIKAWA YUKI, OTANI YUKI, HAYASHI MIKAYO, MIYAJIMA RIN
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY HETEROCYCLIC COMPOUNDS INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	This tetra-functional compound is formed by linking, via a spacer as needed, a ligand binding site (A) or a ligand, a reactive site (D), a potential cleavage site (E), and a biotin site (B), wherein: the ligand binding site (A) is an active functional group such as an amine reactive group or a reactive functional group such as -COOH; the reactive site (D) is a group having a structure of a compound such as 2-aryl-5-carboxy-tetrazole; the potential cleavage site (E) is a group having a structure of a compound such as 1-(4 4-dimethyl-2 6-dioxocyclohex-1-ylidene)ethyl; and the spacer is a group obtained by substitution with a cross-linking group such as a linear or branched alkylene group having 1 or more carbon atoms. 一种四官能性化合物，其根据需要通过间隔物连接配体结合性位点(A)或配体、反应性位点(D)、潜在的可切割位点(E)和生物素位点(B)而形成，其中：所述配体结合性位点(A)是活化的官能团例如胺反应性基团或反应性官能团例如-COOH；所述反应性位点(D)是具有化合物例如2-芳基-5-羧基四唑的结构的基团；所述潜在的可切割位点(E)是具有化合物例如1-(4,4-二甲基-2,6-二氧代环己-1-亚基)乙基的结构的基团；并且所述间隔物是通过用交联基团取代获得的基团，例如具有一个或多个碳原子的直链或支链亚烷基。