Method for detecting content of related substances in fructus dauci carotae or fructus dauci carotae extract

The invention provides a method for detecting the content of related substances in fructus dauci carotae or fructus dauci carotae extract, which comprises the following steps: (a) preparing a test solution, and preparing a reference solution by taking luteolin-7-O-glucuronide as a reference substanc...

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Hauptverfasser: ZHENG XINJIE, ZHANG JINGZE, ZHANG BIQIAN, LIU DAILIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention provides a method for detecting the content of related substances in fructus dauci carotae or fructus dauci carotae extract, which comprises the following steps: (a) preparing a test solution, and preparing a reference solution by taking luteolin-7-O-glucuronide as a reference substance; (b) respectively carrying out high performance liquid chromatography detection on the test solution and the reference solution, wherein the conditions of the high performance liquid chromatography detection are as follows: under the wavelength of 347 nm, octadecylsilane chemically bonded silica is used as a filler of a chromatographic column, a formic acid aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and elution is performed in a gradient mode. According to the method disclosed by the invention, the content of the related substances in the fructus dauci carotae or the fructus dauci carotae extract is detected by adopting the high performance liquid chromatography, so th