Method for constructing specific chromatogram of ophiopogon root decoction

The invention discloses a method for constructing a specific chromatogram of ophiopogon root decoction, which comprises the following steps of constructing a UPLC-UV (Ultra Performance Liquid Chromatography-Ultraviolet) specific chromatogram by taking ammonium glycyrrhizinate, liquiritigenin, liquir...

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Hauptverfasser: TAO CHENLU, ZHU DEQUAN, WEI MEI, FAN QIAN, LUO WEN'AN, CAI SHENGKANG, CHEN XIANGDONG, WU XIAOCHUN, SUN DONGMEI, HE GUANGMING, PAN LIYE
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method for constructing a specific chromatogram of ophiopogon root decoction, which comprises the following steps of constructing a UPLC-UV (Ultra Performance Liquid Chromatography-Ultraviolet) specific chromatogram by taking ammonium glycyrrhizinate, liquiritigenin, liquiritin, isoliquiritin, methylophiopogon japonicus dihydrohomoisoflavone A and methylophiopogon japonicus dihydrohomoisoflavone B as reference substances; by taking ginsenoside Rg1, ginsenoside Re and ginsenoside Rb1 as reference substances, constructing an HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) characteristic spectrum; the method provided by the invention can comprehensively reflect the quality attribute of the traditional decoction of the ophiopogon root decoction, ensures the consistency of the quality of the ophiopogon root decoction preparation and the decoction, and comprehensively and effectively controls the product quality. 本发明公开了一种麦门冬汤特征图谱的构建方法,其包括:以甘草酸铵、甘草素、