Environmental-response type host-guest self-assembled dendritic gene vector as well as preparation method and application thereof

The invention provides an environmental-response type host-guest self-assembled dendritic gene vector as well as a preparation method and application thereof. A guest 1 in which a naphthol group is connected with a three-generation amino dendritic unit is prepared, and a host-guest assembled molecule is spontaneously formed by using a host-guest self-assembly technology, and taking cucurbit [8] uril (CB [8]) as a host and a molecule of a viologen group and polyethylene glycol (PEG) as a guest 2; a self-assembled body can selectively obtain a pH response function according to different connecting functional groups of the guest 2; and the self-assembled body is disassembled in a reducing environment, so as to control the release of the gene. The dendritic molecule is controllable in nanometer size and high in transfection efficiency when being used as a gene vector. 本发明提供一种环境响应型主客体自组装树枝状基因载体及其制备方法和应用。本发明制备萘酚基团与三代氨基树枝状单元相连的客体1，利用主客体自组装技术，以葫芦[8]脲(CB[8])作主体，以紫精基团与聚乙二醇(PEG)的分子作为客体2，自发形成主客体组装分子；自组装体根据客体2的连接官能团不同，可选择