Liquid-based cell preservation liquid and preparation method thereof

The invention discloses a liquid-based cell preservation fluid and a preparation method thereof. The preservation fluid is prepared from the following components in percentage by mass: 15 to 20 percent of alcohol, 0.4 to 0.7 percent of buffer component, 0.2 to 0.5 percent of ionic strength regulator...

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Bibliographische Detailangaben
Hauptverfasser: LEI SUWEI, WU RUNFENG, JIANG XIWEN, JIAN JUNXING, DIAO XUE, QI WENCHUANG
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a liquid-based cell preservation fluid and a preparation method thereof. The preservation fluid is prepared from the following components in percentage by mass: 15 to 20 percent of alcohol, 0.4 to 0.7 percent of buffer component, 0.2 to 0.5 percent of ionic strength regulator, 0.05 to 2 percent of anticoagulant, 0.05 to 0.2 percent of mucus dissociation agent, 0.5 to 0.9 percent of preservative and the balance of purified water. The cell preservation fluid is reasonable in component arrangement, and can preserve cells at normal temperature for a long time and maintain cell morphology; the mucus dissociation agent is added, so that cell mucus can be effectively softened, cells adhered by the mucus in a sample can be separated out, and cell accumulation is avoided; the preservative is added, so that the proliferation of mould, bacteria or other pathogens can be effectively inhibited, and the cell preservation is facilitated. 本发明公开了一种液基细胞保存液及其配制方法;按照质量百分比,该保存液包括15~20%醇类、0.4~0.7%缓冲成分、0.2~0