Method for determining anthelmintic residues in feed by liquid chromatography-tandem mass spectrometry

The invention discloses a method for determining anthelmintic residues in feed by liquid chromatography-tandem mass spectrometry, which comprises the following steps of extracting drugs in a feed sample by acetonitrile or methanol, degreasing by n-hexane, purifying by an HLB solid-phase extraction column, and determining by liquid chromatography-tandem mass spectrometry. Various different types of feed samples are detected, existing detection methods for four abamectin drugs in feed at home and abroad are optimized and improved, detection of milbemycins and moxidectin is added, detection of the content of one or more drugs in multiple feed types and varieties can be achieved, and the method has the advantages of high detection efficiency, good sensitivity, good repeatability and high recovery rate. 本发明公开了液相色谱串联质谱测定饲料中驱虫药残留的方法，包括：饲料样品中的药物经乙腈或甲醇提取，正己烷去脂结合HLB固相萃取柱净化后，采用液相色谱-串联质谱法测定。本发明对多种不同类型的饲料样品进行检测，对国内外现有的饲料中四种阿维菌素类药物的检测方法进行了优化和改进，并增加了对米尔贝霉素和莫西菌素的检测，能够实现对多个饲料种类和品种中单个或多个药物含量的检测，检测效率高，灵敏度佳，重复性好，回收率高。