Synthetic method of dominant malachite green hapten and application of hapten

The invention provides a synthetic method of a dominant malachite green hapten and application of the hapten, and the synthetic method comprises the following steps: coupling 4-formyl phenylboronic acid and acrylic acid under rhodium catalysis to obtain 3-(4-formyl-phenyl)-propionic acid, reacting the compound with N,N-dimethylaniline in the presence of zinc chloride to obtain a recessive malachite green hapten, conducting oxidizing with tetrachloro p-benzoquinone to obtain a dominant malachite green hapten, and cooupling the hapten with carrier protein to obtain the artificial antigen of the dominant malachite green. Experimental results show that the antibody generated by immunizing animals with the antigen is high in specificity and sensitivity, the titer of the obtained antiserum can reach 106, and the detection limit is 0.1 ug/L. 本发明提供一种显性孔雀石绿半抗原的合成方法及其半抗原的应用，包括如下步骤：4-甲酰基苯硼酸和丙烯酸在铑催化下偶联，得到3-（4-甲酰基-苯基）-丙酸，该化合物在氯化锌存在下与N，N-二甲基苯胺反应，得到隐性孔雀石绿半抗原，在通过四氯对苯醌氧化得到显性孔雀石绿半抗原，该半抗原与载体蛋白偶联即得到显性孔雀石绿人工抗原。实验结果表明，采用本发明的抗原免疫动物