Kit for detecting nucleic acid cytosine deaminase APOBEC3B and application thereof

The invention provides a single-stranded DNA (deoxyribonucleic acid), a kit for detecting nucleic acid cytosine deaminase APOBEC3B and application of the kit. The kit is combined with fluorescence detection to sensitively and stably detect the activity of APOBEC3B with different concentrations, more importantly, when the kit is applied to screening of inhibitors, detection of false positive inhibitors can be avoided, and the kit is convenient, rapid, relatively low in price, small in raw material dosage and capable of screening APOBEC3B inhibitors in a high-throughput mode. And the expression level of APOBEC3B in different tissues and cells can be detected. 本发明提供了一种单链DNA，以及一种用于检测核酸胞嘧啶脱氨酶APOBEC3B的试剂盒及其应用。本发明的试剂盒结合荧光检测能够灵敏且稳定地检测出不同浓度APOBEC3B的活性，更重要的是应用在筛选抑制剂上，可以避免检出假阳性抑制剂，且方便快捷、价格较低、原材料用量小，可以高通量筛选APOBEC3B抑制剂。并且能检测出不同组织、细胞中APOBEC3B的表达水平。