Nitric oxide infrared fluorescent probe as well as preparation method and use method thereof

The invention discloses a nitric oxide infrared fluorescent probe as well as a preparation method and a use method thereof. The molecular formula of the nitric oxide infrared fluorescent probe is as shown in a structure (I). The probe is a D-A-D type fluorescent probe constructed by taking o-phenylenediamine modified [c] [1, 2, 5] thiadiazole-5, 6-diamine as an NO recognition group and an electron acceptor and taking a fluorene derivative as an electron donor, an o-phenylenediamine structure as the NO recognition group reacts with NO to generate an aza group, and the fluorene derivative serves as a fluorophore, the probe can generate different fluorescence responses to NO with different concentrations. The probe molecule can accurately indicate the content of nitric oxide, is slightly interfered by other external species, has extremely high selectivity, does not react with other active oxygen and active nitrogen, and is low in cytotoxicity. 本发明公开了一种一氧化氮红外荧光探针及其制备方法和使用方法，所述一氧化氮红外荧光探针的分子式如(Ⅰ)所示的结构；本发明的探针是以邻苯二胺修