In-vitro preservation solution for siniperca chuatsi ovum and application of preservation solution

The invention discloses an in-vitro preservation solution for siniperca chuatsi ovum and application of the in-vitro preservation solution. The preservation solution is prepared from, by volume, 60%-70% of culture solution, 15%-20% of nutrient solution, 9%-23% of fish ovary solution and 1%-2% of ant...

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Hauptverfasser: ZENG KEWEI, WANG SHOURONG, SONG WEN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses an in-vitro preservation solution for siniperca chuatsi ovum and application of the in-vitro preservation solution. The preservation solution is prepared from, by volume, 60%-70% of culture solution, 15%-20% of nutrient solution, 9%-23% of fish ovary solution and 1%-2% of antibacterial solution. The invention further discloses application of the in-vitro preservation solution for the siniperca chuatsi ovum, in-vitro mature siniperca chuatsi ovum is soaked in the in-vitro preservation solution for the siniperca chuatsi ovum under the soaking conditions that each 150-200 siniperca chuatsi ovum is 0.8-1 ml, and after the siniperca chuatsi ovum can be soaked and preserved for 2-3 hours at the temperature of 16-20 DEG C, the fertilization rate is 90% or above, the hatching rate is 89% or above, and the malformation rate is 0.5% or below. The technical problem caused by desynchrony of female and male spawning induction effect time of the siniperca chuatsi is solved. 本发明公开了一种鳜卵子的体外保存液及其应用,保存液