Probe for closing ribosome RNA or globulin RNA in RNA library building process and application thereof

The invention provides a probe for blocking ribosome RNA or globulin RNA in the RNA library building process, the length of the probe is 35-60 nt, and the probe and the ribosome RNA or globulin RNA form a ternary blocking compound through complementary pairing. The rear half region of the probe is i...

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Bibliographische Detailangaben
Hauptverfasser:	LIU QIAN, SONG DONGLIANG, CAO ZHEN, HOU CE, CHEN JINGJING, WANG MAN, JIANG AO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a probe for blocking ribosome RNA or globulin RNA in the RNA library building process, the length of the probe is 35-60 nt, and the probe and the ribosome RNA or globulin RNA form a ternary blocking compound through complementary pairing. The rear half region of the probe is in strict complementary pairing with the target RNA, and 5-9 bases at the 5' end of the probe pairing region are replaced by modified bases; and the front half region of the probe is a pyrimidine base which is used for being embedded into a large groove of a DNA/RNA double strand to form a ternary complex. The 3'-OH of the probe is sealed by MGB for preventing extension of the probe as a primer; and meanwhile, the MGB can be embedded into a small groove of DNA/RNA double strands, so that the stability of the ternary complex is improved. The invention also discloses an application of the gene in rapid removal of ribosome RNA or globulin RNA in RNA library construction. The probe has the advantages of simplicity in op