DNA CONSTRUCT FOR DIAGNOSING AND TREATING CANCER

The present invention relates to a DNA construct, and a strain into which a recombinant vector comprising the DNA construct is introduced. The DNA construct according to the present invention enables the expression levels of genes operably linked downstream of first and second promoters to be balanced in a host strain or cell, so that cancer treatment and diagnosis are performed simultaneously. In addition, the DNA construct of the present invention completely prevents the expression of the anticancer protein and reporter protein when doxycycline is absent so as to regulate the use of doxycycline, thereby enabling the anticancer protein to be expressed in an appropriate amount for cancer treatment and, simultaneously, the size of the cancer to be monitored in real time according to the expression level of the reporter protein. 本发明涉及一种DNA构建体，以及其中已导入了包含该DNA构建体的重组载体的菌株。本发明的DNA构建体允许可操作连接于第一和第二启动子下游的基因的表达水平在宿主菌株或细胞中达到平衡，使得癌症诊断和治疗可以同时进行。此外，本发明的DNA构建体在没有多西环素的情况下，完全不允许抗癌蛋白和报告蛋白表达，因此它能调控多西环素的使用，从而允许抗癌蛋白以用于癌症治疗的适当剂量进行表