Hydrophis cyanocinctus toxin competition method colloidal gold/nanoflower immune lateral flow chromatography detection card

The invention relates to the field of antibody engineering and immunodetection, and provides a Hydrophis cyanocinctus toxin SN311 competition method colloidal gold/nanoflower immune lateral flow chromatography detection card. According to theHydrophis cyanocinctus toxin SN311 competition method coll...

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Bibliographische Detailangaben
Hauptverfasser: WANG RONGZHI, TANG HENGKUN, LING SUMEI, WANG SHIHUA, GAO YEHONG, CHEN RUI, HUANG XIAOTING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the field of antibody engineering and immunodetection, and provides a Hydrophis cyanocinctus toxin SN311 competition method colloidal gold/nanoflower immune lateral flow chromatography detection card. According to theHydrophis cyanocinctus toxin SN311 competition method colloidal gold/nanoflower immune lateral flow chromatography detection card provided by the invention, the detection time is 10 minutes, the detection threshold value (eliminating a T line) of the colloidal gold immune lateral flow chromatography detection card reaches 25 mu g/mL, and the detection limit (vLOD) reaches 0.1 ng/mL; and the detection threshold (eliminating the T line) of the nanoflower immune lateral flow chromatography detection card reaches 25 mu g/mL, and the detection limit (vLOD) reaches 0.01 ng/mL. The colloidal gold/nanoflower immune lateral flow chromatography detection card is prepared by combining a nanoflower material with an anti-Hydrophis cyanocinctus toxin SN311 monoclonal antibody for the f