Multi-marker primer for identifying torreya grandis varieties of torreya grandis yunnanensis, large clove and pans torreya grandis

The invention relates to a molecular characteristic multiple fluorescent SSR marker primer and a method for rapidly identifying new torreya grandis varieties of torreya grandis yunnanensis, large clove and pan torreya grandis. The nucleotide sequence of the molecular characteristic multiple fluoresc...

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Hauptverfasser: ZHANG SUJIONG, LI HAIBO, HU CHUANJIU, SONG QIYAN, CHEN HONGXING, YE BIHUAN, CHEN YOUWU, SHEN JIANJUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a molecular characteristic multiple fluorescent SSR marker primer and a method for rapidly identifying new torreya grandis varieties of torreya grandis yunnanensis, large clove and pan torreya grandis. The nucleotide sequence of the molecular characteristic multiple fluorescent SSR marker primer is as follows: a primer U7: an upstream primer TgU7F: 5 '-FAM-CGCCTTAAACCATGTCCAT-3', and a downstream primer TgU7R: 5 '-AAATTCCAGGAAACAAAGAA-3'; and the primer U734: an upstream primer TgU734F: 5 '-HEX-CTATGAGCGGTGGAACCCTA-3', and a downstream primer TgU734R: 5 '-GTGGGAGAAATGCCACAACT-3'. According to the molecular characteristic multiple fluorescence SSR marker primer, coniferous DNA can be used for simultaneously carrying out rapid early identification on new torreya grandis varieties of torreya grandis yunnanensis, large clove and pan torreya grandis at the same time, and the method is simple, rapid and accurate and is an irreplaceable molecular means for identifying torreya grandis varieti