Detection card, detection primer composition and GICA-RT-LAMP kit for plantago asiatica mosaic virus (PlAMV)

The invention discloses a detection card, a detection primer composition and a GICA-RT-LAMP kit for plantago asiatica mosaic virus (PlAMV), and relates to the technical field of liliaceae crop virus pathogen detection. Serology and nucleic acid detection methods are organically combined, RNA extract...

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Hauptverfasser:	ZHANG YUBAO, QIU YANG, XIE ZHONGKUI, WANG YAJUN, YANG GUO, GUO ZHIHONG, SU XUESI
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a detection card, a detection primer composition and a GICA-RT-LAMP kit for plantago asiatica mosaic virus (PlAMV), and relates to the technical field of liliaceae crop virus pathogen detection. Serology and nucleic acid detection methods are organically combined, RNA extraction is avoided, and the kit has the advantages of being easy and convenient to operate, high in specificity, sensitivity and detection speed, low in cost and free of special instruments and equipment such as a microplate reader and a PCR instrument. Amplification reaction can be completed under the constant-temperature water bath condition, detection results can be directly judged by naked eyes, the kit is suitable for field or on-site rapid screening of the PlAMV and also suitable for real-time accurate monitoring, and reliable technical support is provided for field prevention and treatment, customs inspection and quarantine of the PlAMV. 本发明公开了车前草花叶病毒的检测卡、检测引物组合物、GICA-RT-LAMP试剂盒，涉及百合科作物病毒病原检测技术领域。本发明将血清学和核酸检测方法有