3D tumor holoantigen induced and activated CTL cells in-vitro amplification culture method
The invention provides a 3D tumor holoantigen induced and activated CTL (cytotoxic T lymphocyte) cells in-vitro amplification culture method which comprises the following steps: step 1, on the first day, putting peripheral blood mononuclear cells into a culture medium containing IL-2, lymphoblastic...
Gespeichert in:
| Hauptverfasser: | , , , , |
|---|---|
| Format: | Patent |
| Sprache: | chi ; eng |
| Schlagworte: | |
| Online-Zugang: | Volltext bestellen |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | |
| container_start_page | |
| container_title | |
| container_volume | |
| creator | LIU SHIHONG BI HONGSEN SONG YALI WANG HONG XIU BINGSHUI |
| description | The invention provides a 3D tumor holoantigen induced and activated CTL (cytotoxic T lymphocyte) cells in-vitro amplification culture method which comprises the following steps: step 1, on the first day, putting peripheral blood mononuclear cells into a culture medium containing IL-2, lymphoblastic activating factors and autoserum for culture; step 2, on the third day, adding into a culture medium containing the IL-2, 3D tumor holoantigen and the autoserum, and carrying out induction activation culture; step 3, on the fifth day, adding into a culture medium containing the IL-2, T cell double-signal activating factors and the autoserum, and carrying out activation proliferation culture; and step 4, quantitatively adding a culture medium containing the IL-2, OKT-3 and inactivated fetal calf serum every two days from the seventh day to the fifteenth day, and carrying out multiplication culture on the cytotoxic T cells. According to the method disclosed by the invention, 60-120 times of amplified CTL cells can be |
| format | Patent |
| fullrecord | <record><control><sourceid>epo_EVB</sourceid><recordid>TN_cdi_epo_espacenet_CN113846059A</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>CN113846059A</sourcerecordid><originalsourceid>FETCH-epo_espacenet_CN113846059A3</originalsourceid><addsrcrecordid>eNqNijEKwkAQRbexEPUO4wEChlXRUlbFQqxS2YRhMzEDm52wmc35TeEBLD6Px_tL87ZX0NxLgk6CYFT-UASOTfbUAMZ5XnlCnc1VT_AUwjj3YmJNAtgPgVv2qCwRfA6aE0FP2kmzNosWw0ibH1dme79V7lHQIDWNA3qKpLV7laU97Y-7w_li__l8AeavOsg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>patent</recordtype></control><display><type>patent</type><title>3D tumor holoantigen induced and activated CTL cells in-vitro amplification culture method</title><source>esp@cenet</source><creator>LIU SHIHONG ; BI HONGSEN ; SONG YALI ; WANG HONG ; XIU BINGSHUI</creator><creatorcontrib>LIU SHIHONG ; BI HONGSEN ; SONG YALI ; WANG HONG ; XIU BINGSHUI</creatorcontrib><description>The invention provides a 3D tumor holoantigen induced and activated CTL (cytotoxic T lymphocyte) cells in-vitro amplification culture method which comprises the following steps: step 1, on the first day, putting peripheral blood mononuclear cells into a culture medium containing IL-2, lymphoblastic activating factors and autoserum for culture; step 2, on the third day, adding into a culture medium containing the IL-2, 3D tumor holoantigen and the autoserum, and carrying out induction activation culture; step 3, on the fifth day, adding into a culture medium containing the IL-2, T cell double-signal activating factors and the autoserum, and carrying out activation proliferation culture; and step 4, quantitatively adding a culture medium containing the IL-2, OKT-3 and inactivated fetal calf serum every two days from the seventh day to the fifteenth day, and carrying out multiplication culture on the cytotoxic T cells. According to the method disclosed by the invention, 60-120 times of amplified CTL cells can be</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2021</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211228&DB=EPODOC&CC=CN&NR=113846059A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20211228&DB=EPODOC&CC=CN&NR=113846059A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIU SHIHONG</creatorcontrib><creatorcontrib>BI HONGSEN</creatorcontrib><creatorcontrib>SONG YALI</creatorcontrib><creatorcontrib>WANG HONG</creatorcontrib><creatorcontrib>XIU BINGSHUI</creatorcontrib><title>3D tumor holoantigen induced and activated CTL cells in-vitro amplification culture method</title><description>The invention provides a 3D tumor holoantigen induced and activated CTL (cytotoxic T lymphocyte) cells in-vitro amplification culture method which comprises the following steps: step 1, on the first day, putting peripheral blood mononuclear cells into a culture medium containing IL-2, lymphoblastic activating factors and autoserum for culture; step 2, on the third day, adding into a culture medium containing the IL-2, 3D tumor holoantigen and the autoserum, and carrying out induction activation culture; step 3, on the fifth day, adding into a culture medium containing the IL-2, T cell double-signal activating factors and the autoserum, and carrying out activation proliferation culture; and step 4, quantitatively adding a culture medium containing the IL-2, OKT-3 and inactivated fetal calf serum every two days from the seventh day to the fifteenth day, and carrying out multiplication culture on the cytotoxic T cells. According to the method disclosed by the invention, 60-120 times of amplified CTL cells can be</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2021</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNijEKwkAQRbexEPUO4wEChlXRUlbFQqxS2YRhMzEDm52wmc35TeEBLD6Px_tL87ZX0NxLgk6CYFT-UASOTfbUAMZ5XnlCnc1VT_AUwjj3YmJNAtgPgVv2qCwRfA6aE0FP2kmzNosWw0ibH1dme79V7lHQIDWNA3qKpLV7laU97Y-7w_li__l8AeavOsg</recordid><startdate>20211228</startdate><enddate>20211228</enddate><creator>LIU SHIHONG</creator><creator>BI HONGSEN</creator><creator>SONG YALI</creator><creator>WANG HONG</creator><creator>XIU BINGSHUI</creator><scope>EVB</scope></search><sort><creationdate>20211228</creationdate><title>3D tumor holoantigen induced and activated CTL cells in-vitro amplification culture method</title><author>LIU SHIHONG ; BI HONGSEN ; SONG YALI ; WANG HONG ; XIU BINGSHUI</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN113846059A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2021</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LIU SHIHONG</creatorcontrib><creatorcontrib>BI HONGSEN</creatorcontrib><creatorcontrib>SONG YALI</creatorcontrib><creatorcontrib>WANG HONG</creatorcontrib><creatorcontrib>XIU BINGSHUI</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LIU SHIHONG</au><au>BI HONGSEN</au><au>SONG YALI</au><au>WANG HONG</au><au>XIU BINGSHUI</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>3D tumor holoantigen induced and activated CTL cells in-vitro amplification culture method</title><date>2021-12-28</date><risdate>2021</risdate><abstract>The invention provides a 3D tumor holoantigen induced and activated CTL (cytotoxic T lymphocyte) cells in-vitro amplification culture method which comprises the following steps: step 1, on the first day, putting peripheral blood mononuclear cells into a culture medium containing IL-2, lymphoblastic activating factors and autoserum for culture; step 2, on the third day, adding into a culture medium containing the IL-2, 3D tumor holoantigen and the autoserum, and carrying out induction activation culture; step 3, on the fifth day, adding into a culture medium containing the IL-2, T cell double-signal activating factors and the autoserum, and carrying out activation proliferation culture; and step 4, quantitatively adding a culture medium containing the IL-2, OKT-3 and inactivated fetal calf serum every two days from the seventh day to the fifteenth day, and carrying out multiplication culture on the cytotoxic T cells. According to the method disclosed by the invention, 60-120 times of amplified CTL cells can be</abstract><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext_linktorsrc |
| identifier | |
| ispartof | |
| issn | |
| language | chi ; eng |
| recordid | cdi_epo_espacenet_CN113846059A |
| source | esp@cenet |
| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | 3D tumor holoantigen induced and activated CTL cells in-vitro amplification culture method |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-11T15%3A04%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-epo_EVB&rft_val_fmt=info:ofi/fmt:kev:mtx:patent&rft.genre=patent&rft.au=LIU%20SHIHONG&rft.date=2021-12-28&rft_id=info:doi/&rft_dat=%3Cepo_EVB%3ECN113846059A%3C/epo_EVB%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |