UPLC (Ultra Performance Liquid Chromatography) analysis method of ipratropium bromide aerosol

According to the UPLC analysis method for determining the ipratropium bromide aerosol, the analysis efficiency is remarkably improved, and the detection process is completed within 8 minutes. According to the method, ultra-high performance liquid chromatography is adopted, and the chromatographic conditions are as follows: octadecylsilane chemically bonded silica is used as a filler; a sodium heptanesulfonate aqueous solution (A) with a pH value of 2.6 and acetonitrile (B) with a ratio of 72: 28 are taken as a mobile phase; the detection wavelength is 210 nm; the column temperature is 40 DEG C; the flow velocity is 0.6 ml/min; and isocratic elution is carried out. 本发明提供一种测定异丙托溴铵气雾剂的UPLC分析方法，该方法显著提高分析的效率，检测过程在8分钟内完成。所述方法采用超高效液相色谱法，色谱条件为：用十八烷基硅烷键合硅胶为填充剂；以pH 2.6庚烷磺酸钠水溶液(A)：乙腈(B)＝72：28为流动相；检测波长为210nm；柱温为40℃；流速为0.6ml/min；等度洗脱。