Method for identifying and evaluating resistance level of pleurotus geesteranus to yellow spot disease induced by C.neissei
The invention discloses a method for identifying and evaluating the resistance level of pleurotus geesteranus to yellow spot disease induced by C.neissei. The method comprises the following steps: inoculating a pleurotus geesteranus strain in a cultivation material belt, culturing hyphae for 80 days...
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Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for identifying and evaluating the resistance level of pleurotus geesteranus to yellow spot disease induced by C.neissei. The method comprises the following steps: inoculating a pleurotus geesteranus strain in a cultivation material belt, culturing hyphae for 80 days, and after the hyphae are physiologically mature, stimulating fungus sticks to fruit by temperature difference; carrying out shake culture on a C.neissei strain to obtain a pathogenic bacteria suspension; selecting neat and healthy mushroom sticks with many sporocarps, and conducting inoculation when the size of mushroom caps of the sporocarps is 0.5-1.0 cm; placing in a closed preservation box, and then transferring to a proper temperature and humidity condition for culturing; and after culturing for 2 days, taking out the fungus sticks, investigating the morbidity of the yellow spot disease of the pleurotus geesteranus, recording the number and morbidity level of morbidity sporocarps of the fungus sticks, calcul |
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