Mutant recombinant reverse transcriptase as well as preparation method and application thereof

The invention discloses a mutant recombinant reverse transcriptase as well as a preparation method and application thereof. The specific mutation sites are as follows: the 67th amino acid of a wild reverse transcriptase M-MLV is mutated from S to T, the 303th amino acid is mutated from F to R, the 312th amino acid is mutated from L to S, the 432nd amino acid is mutated from L to G, the 479th amino acid is mutated from N to F, and the 524th amino acid is mutated from D to Q. The transcriptase is obtained through IPTG induced expression by artificially synthesizing an expression vector and utilizing a preferred host cell. The activity temperature of the modified recombinant mutant reverse transcriptase is 37-65 DEG C, the reverse transcription efficiency of the modified recombinant mutant reverse transcriptase is 41.9 times that of a wild reverse transcriptase, and the endurance capacity of the modified recombinant mutant reverse transcriptase to common interferents is obviously improved. The performance of the