Preparation method and application of porcine circovirus type 4 (PCV4)

The invention provides a preparation method and application of a porcine circovirus type 4 (PCV4). A gene sequence of the PCV4 is obtained by inserting regulatory sequences into upstream and downstream of a PCV4 whole genome sequence as shown in SEQ ID NO.4, the regulatory sequence as shown in SEQ ID NO.2 is located on the upstream of the PCV4 whole genome sequence, and the regulatory sequence as shown in SEQ ID NO.3 is located on the downstream of the PCV4 whole genome sequence. The PCV4 virus sample prepared for the first time can be used for research of PCV4 infection and pathogenic mechanisms, vaccine research and development and the like, provides a theoretical basis for epidemic diseases caused by PCV4 infection and an effective tool for preventing and controlling the epidemic diseases, and has wide application prospects in clinical and laboratory research. 本发明提供猪圆环病毒4型制备方法及其应用，猪圆环病毒4型基因序列是在如SEQ ID NO.4所示的PCV4全基因组序列上游和下游插入调控序列得到，如SEQ ID NO.2所示的调控序列位于PCV4全基因组序列上游，如SEQ ID NO.3所示的调控序列位于PCV4全基因组序列下游。本发明首次制备