Superbright monochromatic upconversion nanoprobe for excitation/emission in biological window as well as preparation method and application of superbright monochromatic upconversion nanoprobe

The invention discloses a superbright monochromatic upconversion nanoprobe for excitation/emission in a biological window as well as a preparation method and application of the superbright monochromatic upconversion nanoprobe, and belongs to the technical field of preparation of rare earth luminescent nanomaterials and biological living body imaging. The nanoprobe provided by the invention is a nano-particle which is formed by co-doping Nd, Yb, Er and Mn and has a multi-layer core-shell structure, and the chemical composition of the nanoprobe is alpha-NaYF4:Yb/Er/Mn coated CaF2:Yb coated NaNdF4:Yb. Through component optimization and structural design, the prepared up-conversion probe material obtains high-quantum-efficiency single-band red light emission under the excitation of 808nm near-infrared light. The up-conversion fluorescent probe material is unique in structure and stable in performance, has the advantages of high tissue penetration depth, high sensitivity, high resolution, high sign