Molecular marker for identifying beef components and application thereof

The invention discloses a molecular marker for identifying beef components and application thereof, and a beef component detection method with strong specificity and high sensitivity is established by using a cattle single copy gene in a cell nucleus as a molecular marker through a real-time fluorescent quantitative PCR technology based on SYBR Green I dye. According to the method established by the invention, cattle can be accurately distinguished from other 12 animals and 6 plants, 9 meat products on the market are actually detected, and whether the cattle-derived components are contained or not can be accurately judged. The bovine specificity real-time fluorescent quantitative PCR detection method established by the invention can provide technical reference for qualitative and quantitative detection of bovine-derived components in mixed meat products. 本发明公开一种鉴定牛肉成分的分子标记及其应用，通过基于SYBR Green I染料的实时荧光定量PCR技术，利用细胞核中牛的单拷贝基因作为分子标记，建立具有特异性强、灵敏度高的牛肉成分检测的方法。本发明建立的方法可以准确区分牛与其他12种动物和6种植物，对9种市场上的肉制品进行实际检测，可准确判断出是否含有牛源性