Method for improving conversion efficiency of exogenous DNA to corn

The invention discloses a method for improving the conversion efficiency of exogenous DNA (deoxyribonucleic acid) to corn. The method comprises the following steps of after artificial pollination of corn, removing inner bracts and all filaments of a female ear, sleeving the female ear with a sealed container, fixing the female ear with the sealed container, reversing the whole corn plant, fixing or suspending the whole corn plant, adding an exogenous DNA solution into the sealed container, soaking the female ear without filaments in the DNA solution, and finally restoring the corn plant. The method provided by the invention has the advantages of long soaking time of the exogenous DNA solution, short introduction path and capability of fully utilizing a pollen tube channel to improve the conversion efficiency of the exogenous DNA to the corn, and is easy to operate and low in cost. 本发明公开了一种提高外源DNA转化玉米效率的方法。在玉米人工授粉后，除去雌穗里层苞叶及全部花丝，套上密封的容器，并固定好，将整个玉米植株倒转过来，固定或悬挂，在密封的容器内加外源DNA溶液，让无花丝的雌穗浸泡在DNA溶液中，最后复原玉米植株。本发明是一种外源D