Method for obtaining filial generation of cultivated tobacco and nicotiana alata
The invention provides a method for obtaining filial generation of cultivated tobacco and nicotiana alata. The method comprises the following steps: taking pollen of first cultivated tobacco, and then performing induction treatment on stigmas of second cultivated tobacco by using the pollen of the f...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a method for obtaining filial generation of cultivated tobacco and nicotiana alata. The method comprises the following steps: taking pollen of first cultivated tobacco, and then performing induction treatment on stigmas of second cultivated tobacco by using the pollen of the first cultivated tobacco to obtain stigmas subjected to first treatment; sequentially carrying out cell disruption treatment, drying treatment and mashing treatment on the stigmas subjected to the first treatment to obtain stigmas subjected to second treatment; taking pollen of the nicotiana alata, and mixing the pollen of the nicotiana alata with the stigmas subjected to second treatment according to a preset proportion to obtain a mixture to be treated; repeatedly pollinating the third cultivated tobacco for multiple times within 120 hours after the initial opening period through the to-be-treated mixture to obtain the pollinated cultivated tobacco; and carrying out detection operation and screening operation on t |
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