PET hydrolase IsPETase mutant enzyme, coding gene and engineering bacterium

The invention discloses a PET hydrolase IsPETase mutant enzyme, a coding gene and an engineering bacterium. The PET hydrolase IsPETase mutant enzyme is named as IsPETaseR280A/S121K-cSP, and the amino acid sequence of the IsPETaseR280A/S121K-cSP is as shown in SEQ ID NO.1. According to the invention,...

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Bibliographische Detailangaben
Hauptverfasser:	YIN QINGDIAN, QI WEI, SU RONGXIN, YOU SHENGPING
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses a PET hydrolase IsPETase mutant enzyme, a coding gene and an engineering bacterium. The PET hydrolase IsPETase mutant enzyme is named as IsPETaseR280A/S121K-cSP, and the amino acid sequence of the IsPETaseR280A/S121K-cSP is as shown in SEQ ID NO.1. According to the invention, a rational design technology is adopted to perform molecular modification on PET hydrolase IsPETase, the PET hydrolase IsPETase mutant enzyme, namely the IsPETaseR280A/S121K-cSP, with improved hydrolysis efficiency is obtained by methods such as signal peptide prediction and homologous modeling, so that the hydrolysis efficiency of PET is improved, and besides, the PET hydrolase IsPETase mutant enzyme and MHETase-cSP are combined to improve the hydrolysis efficiency of PET. 本发明公开了PET水解酶IsPETase突变酶及编码基因及工程菌，PET水解酶IsPETase突变酶，命名为IsPETaseR280A/S121K-cSP，所述IsPETaseR280A/S121K-cSP的氨基酸序列如SEQ IDNO.1所示。本发明采用理性设计技术对PET水解酶IsPETase进行分子改造，经过信号肽预测、同源建模等方法而获得水解效率提高的PET水解酶IsPETase突变酶即IsPETaseR280A/S121K-cSP，使对PET水解效率提高，同时，采用PET水