Preparation and application of active bacterium Bacillus sp for inhibiting tobacco mildew rot

The invention discloses preparation and application of active bacteria Bacillus sp for inhibiting the tobacco mildew rot, and relates to the technical field of microbial pesticides. A preparation process of the active bacteria Bacillus sp for inhibiting the tobacco mildew rot comprises the steps: inoculating NL-6 bacteria into a test tube LB liquid culture medium (5 ml per tube), wherein a formula of the culture medium comprises 0.5% of yeast extract, 1% of peptone, and 1% of NaCl; and inoculating the NL-6 thalli onto the culture medium, and culturing in a shaking table at the temperature of 37 DEG C for 12 hours at the rotating speed of 220rpm to obtain a test tube strain. The active bacterium Bacillus sp has the inhibitory activity on various pathogenic bacteria of the tobacco mildew rot, and after the activity of the active bacterium Bacillus sp is determined through multiple batches of activity tests for inhibiting the various pathogenic bacteria of the tobacco mildew rot, the strain is subjected to morph