Preparation method of insulin aspart

The invention provides an insulin aspart derivative and a preparation method thereof. Specifically, according to the method, insulin aspart fusion protein containing a green fluorescent protein folding unit is expressed in escherichia coli in a high-density mode, and enzyme digestion and purification are conducted on the fusion protein to prepare the insulin aspart. The method disclosed by the invention does not need to be carried out in a solid-phase organic system, process steps are reduced, environmental pollution is small, cost is lower, and the method is suitable for popularization. 本发明提供了一种门冬胰岛素衍生物及其制备方法。具体地，本发明的方法在大肠杆菌中高密度表达包含绿色荧光蛋白折叠单元的门冬胰岛素融合蛋白，并对该融合蛋白进行酶切和纯化，制备门冬胰岛素。本发明的方法无需在固相有机体系下进行，减少工艺步骤，对环境污染小，成本更低，适宜推广。