Micro-fluidic chip, preparation method thereof and application of micro-fluidic chip in C-reactive protein detection by combining two-step microsphere competition method

The invention discloses a micro-fluidic chip, a preparation method thereof and an application of the micro-fluidic chip in C-reactive protein detection by combining a two-step microsphere competition method.The micro-fluidic chip comprises a PDMS chip located at the top, a PDMS film in the middle and a substrate at the bottom, micro-channels or micro-channel arrays of different shapes, a solution outlet and a solution inlet are formed in the PDMS chip, the solution inlet is located at the starting end of the micro-channel or the micro-channel array, the solution outlet is located at the tail end of the micro-channel or the micro-channel array, and the micro-fluidic chip can quantify the number of the microspheres with naked eyes and directly read the number of the microspheres. According to the application of the micro-fluidic chip in C-reactive protein detection in combination with a two-step microsphere competition method, high-precision quantification of biomarkers with the concentration range of 10 pg/mL