SSR primer pair for identifying variety of Chinese ornamental peony and variety molecular identification method

The invention discloses an SSR primer pair for identifying the variety of Chinese ornamental peony and a variety molecular identification method. The invention provides 10 pairs of high-polymorphism SSR primers, the PIC value is 0.77-0.86, the average value is 0.804, and 3-10 pairs of primers can be...

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Bibliographische Detailangaben
Hauptverfasser: WAN YINGLING, LIU YAN, LIU AIQING
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses an SSR primer pair for identifying the variety of Chinese ornamental peony and a variety molecular identification method. The invention provides 10 pairs of high-polymorphism SSR primers, the PIC value is 0.77-0.86, the average value is 0.804, and 3-10 pairs of primers can be used for identifying the variety of the Chinese ornamental peony. 221 Chinese herbaceous peony varieties can be distinguished by using 3 pairs of primer combinations, 352 varieties can be distinguished by using 6 pairs of primer combinations, 397 varieties can be distinguished by using 9 pairs of primer combinations, and 403 varieties participating in the test can be distinguished by using the 10 pairs of primer combinations. According to the SSR primer pair for identifying the variety of the Chinese ornamental peony and the variety molecular identification method, the 10 pairs of primers have efficient identification capability on the Chinese herbaceous peony varieties. Meanwhile, by using the multiple capillary