mRNA-based vaccine against coronavirus and preparation method thereof

The invention relates to the field of medicine, in particular to an mRNA (messenger ribonucleic acid)-based vaccine aiming at coronavirus and a preparation method thereof. The vaccine comprises a liposome-coated mRNA-RBD+NTD+RBD vaccine; a mRNA is designed according to the genome of Venezuela equine encephalomyelitis virus TC83 in the [alpha] virus family; the mRNA-RBD+NTD+RBD contains a gene which secretes a signal peptide and a new coronal pneumonia Spike protein gene receptor binding region (RBD)+N-terminal region (NTD)+receptor binding region (RBD)+Foldon sequence; and according to the invention, the antigen of the coronavirus can be expressed by using one mRNA, so that the immunogenicity is strong, the pain of a patient can be relieved, and the immune effect can be achieved. 本发明涉及医药领域，具体涉及基于mRNA的针对冠状病毒的疫苗及其制备方法，疫苗包括：脂质体包裹的mRNA-RBD+NTD+RBD的疫苗；mRNA根据α病毒家族中委内瑞拉马脑脊髓炎病毒TC83的基因组设计而成；mRNA-RBD+NTD+RBD包含了分泌信号肽，新冠肺炎刺突Spike蛋白基因受体结合区(RBD)+N端区(NTD)+受体结合区(RBD)+Foldon序列的基因；本发明通过使用一条mRNA能够表达冠状病毒的抗原，具有强免疫原性，既能减轻病人痛苦，又能够达