miRNA kit for diagnosing adenomyosis

The invention belongs to the technical field of biology, and particularly relates to a molecular diagnosis technology for adenomyosis. According to the technical scheme, the miRNA kit for diagnosing adenomyosis comprises any combination of reverse transcription primers of hsa-miR-22-3p, hsa-miR-182-...

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Hauptverfasser: WAN GUIPING, ZHUANG NANFEI, ZHANG LEI, XIA MEIQI, CHENG CHEN, YANG GUIYUN, XING YUXIA, XUN AIHUA, ZHU LI, GUI TAO, ZHANG LIHUA, XU AIYUN, HUANG MEIHUA, NI YONGYAN, GAN LIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention belongs to the technical field of biology, and particularly relates to a molecular diagnosis technology for adenomyosis. According to the technical scheme, the miRNA kit for diagnosing adenomyosis comprises any combination of reverse transcription primers of hsa-miR-22-3p, hsa-miR-182-5p and hsa-miR-103a-3p, a reagent for a probe method qPCR reaction, namely a probe and forward and reverse PCR primers. The kit can also comprise a single-chain RNA standard substance of the hsa-miR-22-3p, the hsa-miR-182-5p and the hsa-miR-103a-3p. A serum miRNAs expression database and a specific marker, namely any combination of hsa-miR-22-3p, hsa-miR-182-5p and hsa-miR-103a-3p, related to adenomyosis incidence are obtained through a large amount of deep research in the early stage. Then any two of the hsa-miR-22-3p, the hsa-miR-182-5p and the hsa-miR-103a-3p are detected through development and application of the specific diagnostic kit. As a diagnostic specificity index, the diagnosis of adenomyosis can be mor