miRNA kit for diagnosing adenomyosis
The invention belongs to the technical field of biology, and particularly relates to a molecular diagnosis technology for adenomyosis. According to the technical scheme, the miRNA kit for diagnosing adenomyosis comprises any combination of reverse transcription primers of hsa-miR-22-3p, hsa-miR-182-...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention belongs to the technical field of biology, and particularly relates to a molecular diagnosis technology for adenomyosis. According to the technical scheme, the miRNA kit for diagnosing adenomyosis comprises any combination of reverse transcription primers of hsa-miR-22-3p, hsa-miR-182-5p and hsa-miR-103a-3p, a reagent for a probe method qPCR reaction, namely a probe and forward and reverse PCR primers. The kit can also comprise a single-chain RNA standard substance of the hsa-miR-22-3p, the hsa-miR-182-5p and the hsa-miR-103a-3p. A serum miRNAs expression database and a specific marker, namely any combination of hsa-miR-22-3p, hsa-miR-182-5p and hsa-miR-103a-3p, related to adenomyosis incidence are obtained through a large amount of deep research in the early stage. Then any two of the hsa-miR-22-3p, the hsa-miR-182-5p and the hsa-miR-103a-3p are detected through development and application of the specific diagnostic kit. As a diagnostic specificity index, the diagnosis of adenomyosis can be mor |
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