Manufacturing of medical fulvic acid standard substance and application of medical fulvic acid standard substance in cell experiment

The method comprises the following steps: carrying out catalytic oxidation on peat, lignite and weathered coal humic acid, carrying out deep separation through a vertical cyclone machine, a horizontal spiral separator and a disc centrifuge, carrying out gel phase change deliming, carrying out continuous desalination through a water-alcohol membrane, a homogeneous phase and a heterogeneous electrodialysis membrane, and carrying out molecular weight determination to obtain the medical fulvic acid standard product. An inhibition experiment on transaminase of hepatoma carcinoma cells Hep1-3B is carried out. The fulvic acid can react with alpha-glucosidase, experiments for preventing pancreatic resistance and reducing blood sugar and survival rate in human brain glioma U87 cells prove that the drug effect reaches 35.28-80.00% or above, the fulvic acid has an adjusting effect on brain barriers, the additional value of fulvic acid pharmaceuticals is effectively improved, and the application prospect is wide. 本发明通过对泥