Fluorescence detection system and fluorescence spectrum splicing method

The invention provides a fluorescence detection system and a fluorescence spectrum splicing method, wherein the fluorescence detection system adopts a dichroic film to split light according to wavelength, and through combination of a plurality of fluorescence detection modules, not only can an interested wave band be extracted, but also a fluorescence signal can be collected in a visible light full spectrum range through splicing; meanwhile, the system has high spectral wavelength resolution, high sampling rate and readout rate, and is particularly suitable for collection and detection of weak fluorescence; and according to the fluorescence spectrum splicing method, the processing module can correct the spectral irradiance of the photomultiplier at the spectrum splicing position of each fluorescence detection module, spectral information loss caused by dichroic film light splitting can be restored, the accuracy and continuity of spectrum collection of the system are guaranteed, and the method is especially su