Fluorescence immunodetection system

The invention provides a fluorescence immunodetection system. The system comprises a micro-fluidic chip and a portable analysis device, the protein marker substrate is pretreated to form a multi-index biological array; the chip shell is respectively provided with a sample pool, a driving piston, a piston driving pool, a reaction pool, a cleaning pool and a detection area; the analysis evice is used for assisting the substrate in completing biological reaction in the micro-fluidic chip and carrying out signal acquisition and result analysis. The bottom end of the piston driving pool is connected with the top end of the sample pool, sample liquid enters the reaction pool to react with the substrate through the micro-fluidic structure under the motion of the piston, the substrate is controlled by an instrument to complete cleaning and fluorescence marker capturing in the chip, and collection of a substrate fluorescence signal is completed in a detection area. The analysis device realizes automatic realization of