Molecular characteristic SSR primer and method for identifying torreya grandis polyploidy variety

The invention relates to a molecular characteristic SSR marker primer for rapidly identifying a torreya grandis polyploidy variety and a method for identifying a torreya grandis polyploidy. The nucleotide sequence of the molecular characteristic fluorescent SSR primer is as follows: a primer TgU33:...

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Bibliographische Detailangaben
Hauptverfasser: ZHANG SUJIONG, LI HAIBO, HU CHUANJIU, SONG QIYAN, CHEN HONGXING, YE BIHUAN, CHEN YOUWU, SHEN JIANJUN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to a molecular characteristic SSR marker primer for rapidly identifying a torreya grandis polyploidy variety and a method for identifying a torreya grandis polyploidy. The nucleotide sequence of the molecular characteristic fluorescent SSR primer is as follows: a primer TgU33: an upstream primer TgU33F: 5 '-FAM-TAAGAGGATTGACATGGCC-3', a downstream primer TgU33R: 5 '-TATTCTGGAGTTTGGACCCG-3'. According to the molecular characteristic SSR marker primer for rapidly identifying the torreya grandis polyploidy variety and the method for identifying the torreya grandis polyploidy, the molecular characteristic fluorescent SSR primer can be used for rapidly identifying the torreya grandis polyploidy variety by using coniferous DNA, the method is simple, rapid, accurate and efficient, the repeatability of a detection result is good, the method is not influenced by seasons, plant development periods and growth environments. Reference and basis are not only provided for the rapidly identifying of the