Method for quantitatively detecting IFN-gamma concentration by using chemiluminescence method and detection kit thereof

The invention discloses a method for quantitatively detecting IFN-gamma concentration by using a chemiluminescence method, which comprises the following steps: on the basis of a ligand-receptor binding principle, binding a coating protein, a detection substance IFN-gamma and a detection antibody on a solid medium in sequence, and then adding a chromogenic substrate for detection, the detection method comprises the following steps: fixing a specific protein of IFN-gamma by using a solid medium, wherein the specific protein is a coating protein, and then adding a detection sample, specifically fixing the IFN-gamma in the sample on the solid medium, then fixing a second antibody, namely a detection antibody, which can be combined with IFN-gamma on the fixing medium, washing away the non-specifically adsorbed impurities along with the washing process; specially marking the second antibody directly or indirectly, and finally, using a substrate adaptive to the marker for color development detection. The method has