Method for constructing in-vitro foam cell formation model in atherosclerosis process

The invention relates to the technical field of cell model construction methods, in particular to a method for constructing an in-vitro foam cell formation model in an atherosclerosis process, which comprises the following steps: culturing endothelial cells and macrophages in a Transwell chamber to obtain a two-cell co-culture system; adding tNF-alpha and ox-LDL into the two-cell co-culture systems to obtain a foam cell induction system, and the foam cell induction system is cultured to obtain a cell culture system containing foam cells, namely the foam cell formation model. The in-vitro atherosclerosis early formation process can be simulated through the foam cell formation model, compared with an existing model construction method, the method for constructing the foam cell formation model can obviously reduce the modeling time and expenditure consumption, and a new alternative in-vitro cell screening model is provided for the atherosclerosis diagnosis and treatment process. 本发明涉及细胞模型构建方法技术领域，是一种构建动脉粥样硬化过程中体