Method for subpackaging cells in cell bank building process

The invention discloses a method for subpackaging cells in a cell bank building process. The method comprises the following steps: assembling a liquid dispenser sterilization and disinfection assembly, wherein the liquid dispenser sterilization and disinfection assembly is packaged in a sterilizatio...

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Bibliographische Detailangaben
Hauptverfasser: SUI LILI, HU DICHAO, KONG LINGJIE
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention discloses a method for subpackaging cells in a cell bank building process. The method comprises the following steps: assembling a liquid dispenser sterilization and disinfection assembly, wherein the liquid dispenser sterilization and disinfection assembly is packaged in a sterilization sealing bag and is subjected to high-pressure sterilization; enabling the liquid dispenser sterilization and disinfection assembly and a cell culture shake flask with the packaging bag to be subjected to ultraviolet radiation disinfection; amplifying and centrifugally collecting cells, and adjusting cell suspension to the required density by using a culture medium; in a biological safety cabinet, removing packages of the liquid dispenser sterilization and disinfection assembly and the cell culture shake flask, transferring the cell suspension into the cell culture shake flask, then taking down the liquid dispenser, and connecting the liquid dispenser with the cell culture shake flask to form a cell suspension spl