Method for synthesizing ester substances through multi-liquid-phase enzymatic esterification

The invention belongs to the field of bioengineering, and discloses a method for synthesizing ester substances through multi-liquid-phase enzymatic esterification. The method comprises the following steps: (1) adding a hydrophilic solvent and soluble salt into lipase liquid, and fully mixing; (2) adding organic alcohol and organic acid with more than C4 into the mixed solution to form a three-liquid-phase system, and carrying out esterification reaction under a stirring condition; and (3) after the reaction is finished, standing or centrifugally layering, and collecting an upper-layer product ester. Different from a water serious inhibition reaction generated in a traditional esterification process, water rich in the three liquid phases does not have an obvious inhibition effect on an esterification reaction, redundant water molecules do not need to be removed under conditions of adding a dewatering agent or vacuum and the like in the esterification process, and operation complexity is reduced. The method has